This application documents how we performed a stable, no-wash cytokine experiment using the Spectramax Paradigm Multi-Function Reader with a good Z factor.
HTRF is a versatile technology developed by Cisbio Reagents to detect biomolecular interactions. It combines fluorescence resonance energy transfer (FRET) technology with time-resolved fluorescence (TRF) technology to eliminate background fluorescence with short half-lives. This experiment uses donor and acceptor fluorescent dyes. When the donor and the acceptor are close enough, the donor excitation light generated by illumination by a light source (eg, a high-energy scintillation xenon lamp) passes through an energy transfer mechanism to the receptor, which in turn acts as a specific excitation wavelength for the receptor. HTRF uses four specific fluorescent dyes that can be combined into a compatible donor-acceptor TR-FRET pair. The donors are lanthanum (Eu3+) and lanthanum (lumi4TM-Tb), and their long-lived fluorescence allows them to be used in time-resolved fluorescence experiments. Two receptors have been developed for HTRF experiments, XL665 and d2, respectively. Both have an emission spectrum of the HTRF donor with excitation spectra overlapping. Each has an emission peak at 665 nm, but the wavelength of this region does not excite the donor, or the excitation is very weak. The earliest HTRF receptor, XL665, was purified from the phycobiliprotein pigment of red algae. The second-generation receptor, d2, is a modified allophycocyanin, 100 times smaller than the XL665, to alleviate the steric hindrance that may occur during XL665 detection.
Salted Shredded Kelp
The so-called original ecological kelp means that we pack the young kelp with saturated salt water directly after washing without any preservatives.Original ecological products are the favourite of housewives.
How to eat:
- Soaking about 30 minutes to moderate salty
- Cook . make salad or add to hot pot after draining the wate
The difference between deep sea kelp and traditional ordinary kelp
Nutrational Effects
Salted Shredded Kelp,Shredded Seaweed,Wakame Kelp,Organic Sea Kelp
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