1. The sample is best used now, or within a few days of -35 degrees, the thawed sample should be thoroughly mixed and used.
2. Samples with high levels of hemolysis and blood lipids should not be used. If you need to save for a long time, quickly freeze in a -35 degree refrigerator to prevent repeated freezing and thawing.
3. The insulin is in a stable state at 2-8 degrees, and a final concentration of 100-500 KIU/ml of aprotinin is required for storage.
Domestic brand, item number A8260, size 5mg, 1mg> 4500KIU, price 260
Storage: 5mg of stock solution is reconstituted with 500ul water, final concentration 10mg/ml, pH7-8
Storage conditions: 2-8 ° C
Method of use: Add 1 ul of serum or anticoagulated plasma, 5 mg can process 50 ml of sample
Product: C-peptide ELISA Kit
1. The sample is best used now, or within a few days of -35 degrees, the thawed sample should be thoroughly mixed and used.
2. Samples with high levels of hemolysis and blood lipids should not be used. If you need to save for a long time, quickly freeze in a -35 degree refrigerator to prevent repeated freezing and thawing.
3. The insulin is in a stable state at 2-8 degrees, and a final concentration of 100-500 KIU/ml of aprotinin is required for storage . The final pH range is 6.5-7.5 .
Product: Ultra Sensitive Mouse Insulin ELISA Kit
Sample preparation:
1. Plasma collection: After collecting blood, add anticoagulant (final concentration 1 unit/mL heparin, 0.1% EDTA or 0.76% sodium citrate), centrifuge at 4 degrees 2000g for 20min .
2. Serum collection: Blood was collected and centrifuged at 4 ° 2000 g for 20 min after blood coagulation was completed.
Product: Rat Adiponectin ELISA Kit
Sample preparation:
1. Plasma collection: After collecting blood, add anticoagulant (final concentration 1 unit/mL heparin or 0.1% EDTA), centrifuge at 4 degrees 2000g for 20min .
2. Serum collection: Blood was collected and centrifuged at 4 ° 2000 g for 20 min after blood coagulation was completed.
3. The sample can be stored at room temperature for 2 days and stored at -20 degrees for 2 years.
Product: Rat GLP-1 ELISA Kit
Sample preparation:
1. Plasma collection: After collecting blood, add 1 mg/mL EDTA-2Na anticoagulant and centrifuge at 4 degrees 2000g for 20min.
2. Serum collection: Blood was collected and centrifuged at 4 ° 2000 g for 20 min after blood coagulation was completed.
3. Store samples at -70 degrees to prevent repeated freezing and thawing.
1. Serum collection: blood is taken by venipuncture, and the blood is agglutinated after centrifugation. Avoid placing the sample at room temperature or 2-8 degrees for more than 2 hours, and store it at -80 degrees for a long time to avoid repeated freezing and thawing.
2. Plasma collection: blood was taken by venipuncture, EDTA anticoagulant was added to the collection tube, and centrifuged. Avoid placing the sample at room temperature or 2-8 degrees for more than 2 hours, and store it at -80 degrees for a long time to avoid repeated freezing and thawing. If the BD P800 collection tube (anticoagulation collection tube) is used , the stability of the sample will be increased by 20-30%, the room temperature can be placed for 6 hours, and the 2-8 degrees can be repeatedly frozen and thawed 4 times.
3. Adding aprotinin does not increase sample stability .
4. Cell culture fluid: Note that the chemical composition of the culture fluid may affect the analytical results, such as NaN3 and B-mercaptoethanol.
1. Serum collection: blood is taken by venipuncture, and the blood is agglutinated after centrifugation.
2. Plasma collection: blood was taken by venipuncture, heparin or EDTA anticoagulant was added to the collection tube, and centrifuged.
3. The sample can be stored at 2-8 degrees for 24 hours and stored at -20 degrees for a long time to avoid repeated freezing and thawing.
1. Serum collection: The collected blood was agglutinated in a centrifuge tube for 30 min at room temperature, 2000-3000 g, and centrifuged at 4 degrees for 15 min. Stored at -70 degrees for a long time.
2. Plasma collection: Blood was collected and added to a final concentration of 1.735 mg/ml K3EDTA and centrifuged. Stored at -70 degrees for a long time.
1. Plasma collection: The collected blood is 30s built into the collection tube containing EDTA, and the required amount of DPP-IV inhibitor is added according to the product instructions.
Storage conditions: 4 ° C
Method of use: 10ul DPP-IV per milliliter of plasma
2. Centrifuge at 4 degrees 1000g for 10min, the sample can be stored for 3h at 4 degrees, and stored at -70 degrees for a long time to avoid repeated freezing and thawing.
Health-preserving Herbal Tea, listen to the name, you can see three meanings. One is to keep in good health, give a person a very healthy feeling; Second, flowers and plants, Chinese traditional medicine treatment is to use some natural flowers and plants to regulate the body; Third, tea. China has been the birthplace of tea culture since ancient times. A warm cup of tea gives people a warm feeling. So what are the benefits of herbal teas?
The reason why health herbal tea can be called health is because it can recuperate people's body deficiencies to a certain extent. For example, regulating the spleen and stomach, nourishing liver and eye protection, promoting blood circulation and removing stasis. Some health care herbal tea long-term drinking, but also can make people produce antibodies, to achieve the anti-cancer effect. As we all know, with the aggravation of pollution and the change of living habits, cancer happens more and more frequently, which is one of the reasons why so many people pay attention to health preservation.
Everyone is in pursuit of health, and health herbal tea is the simplest way. Do not need to spend much energy, take up how much time, spend how much money, how much mind. A cup of warm health herbal tea, in the office, hand can be to, can quench thirst, but also health, why not?
Health Scented Tea,Jasmine Flower Tea,Lemon Scented Tea,Blooming Jasmine Tea
XuChang Youjia Food Product Development Co., LTD , https://www.yjgrain.com