Oxford Nanopore is developing a nanopore device called SmidgIon. The device uses the same nano-sensing technology as the MinION and PromethIon devices, but it is smaller than the MiniION and has 256 channels in each flow cell. It can be connected to a smartphone or other mobile device via a charging interface and a headphone jack for 4-5 hours.
The Oxford Nanopore user group meeting was held in London at the end of May. The media has not been invited, but some users and bloggers have already posted the latest news on Twitter. Oxford Nanopore is developing a smartphone-powered nanopore sequencer and a low-cost library preparation system.
According to Clive Brown, CTO, Oxford Nanopore is developing a nanopore device called SmidgIon. The device uses the same nano-sensing technology as the MinION and PromethIon devices, but it is smaller than the MiniION and has 256 channels in each flow cell. It can be connected to a smartphone or other mobile device via a charging interface and a headphone jack for 4-5 hours.
The SmidgIon device is suitable for a variety of on-site analyses and is expected to be used for remote monitoring of pathogens during disease outbreaks, on-site analysis of environmental samples, real-time identification of food, wood or wildlife, and field analysis of agricultural environments. This product is expected to be launched in the fourth quarter of 2017.
Oxford Nanopore's Zumbador project is developing another simple, portable product that combines nucleic acid extraction and library preparation in one device. In the illustration, this device is inserted directly into the flow cell. The original sample is added at one end and exits the other end for immediate sequencing.
According to Brown, the first PromethIon sequencer was delivered to customers in the near future. He showed some internal data for the platform, using 48 modular flow cells, each containing 3,000 channels. With R9 reagent, its accuracy and pore utilization are consistent with MinION. PromethIon is able to get up to 6.2 Tb of data in every 48 hours of operation and is expected to increase to 12.4 Tb in the future.
In addition, flow cells with R7 nanopores will no longer be sold, but instead will be replaced by flow cells with R9 nanopores. The new flow cell uses a more robust membrane and a new helicase motor protein. Brown said the company's longest read on the new flow cell is 500 kb long and is currently working to improve throughput and homopolymer detection.
According to the company, R9 brings greater accuracy, especially for 1D sequences, where only one strand of DNA is read. The R9 flow cell is sequenced at 250 bases per second, which is a significant improvement over the 70 bases per second of the previous R7, thus greatly increasing the yield of MinION. According to Brown, the accuracy of the current 1D sequence is close to 90%, while the 2D sequence is around 95%.
With the launch of the R9 flow cell, Oxford Nanopore will also introduce a rapid sequencing kit. Its library preparation takes only 10 minutes and requires less consumables than ever before, analyzing the data immediately after the start of the MinION run. Currently, this kit is being tested by some early trial customers and will be launched soon.
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