Single-cell mRNA sequencing reveals the role of premature dendritic cells in inflammatory responses
-- Studies have shown the importance of single-cell sequencing in finding rare cell populations that are usually hidden in massive cell samples
Boston and South San Francisco, California, in June 2014 30-- published this month in Nature article, Broad Institute and FLUIDIGM scientists using single-cell C1 TM automatic preparation system for preparing cells for single-cell sequencing and found A subset of premature cells in a population of dendritic cells stimulated by the same antigen express antiviral genes earlier. These premature cells cannot be detected because they are usually detected in an average amount of cells. Based on the contribution of the FLUIDIGM and Broad Institutes in single-cell genomics, this discovery will allow researchers to explore how these precocious cells regulate the body's immune response when exposed to exogenous attacks.
Nature's article also highlights the role of cellular heterogeneity and its importance in intercellular communication in immune responses. In the usual experiments, cells are studied together in an environment, allowing communication and signaling between cells. However, the unique characteristics of C1 TM FLUIDIGM automatic preparation of a single cell system allows the researcher to find isolated single cells those cells expressing specific antiviral genes. Because these specific cells are isolated, cells that do not express the antiviral gene do not elicit an immune response, suggesting that intercellular communication plays a crucial role in the immune response.
In addition to discovering rare cell subpopulations, this study sets a new standard for data from single-cell studies. The 1774 cell data published in the article is by far the largest single-cell genomic data and the largest single-cell mRNA sequencing data.
In order to discover and describe these rare precocious cells that account for only 1% of the dendritic cell population, researchers need to scale up to a very high number of cells. To achieve this, they first need to address two key questions: how to prepare a single-cell library containing thousands of cells, and how to sequence such a large number of samples within an affordable range.
For the preparation of a great number of single-cell library, researchers rely on the start of the experiment just listed single cell C1 TM automatic preparation system. The microfluidic technology of the C1TM system allows researchers to quickly and reliably separate, process and study single cells for genomic analysis. In the study, scientists found that stable single-cell data can be obtained with a read length of less than 1 million (usually 5 to 10 million read lengths are required for massive mRNA sequencing). The faster time scientists get stable data greatly reduces the cost of running the experiment.
To get the complete article - Single-cell RNA-seq reveals dynamic paracrine control of cellular variation, Nature. 2014 Jun 19;509(7505):363-9
Please visit: http://info.fluidigm.com/Shalek_Et_Al.html
Single cell genomics initiative
In 2012, the Broad Institute's genomics platform and FLUIDIGM (NASDAQ: FLDM) established a single-cell genomics initiative to accelerate the development of mammalian single-cell genomics research methods and the discovery of results. The Single Cell Genomics Initiative is also a bridge between many single-cell genomics researchers in the forefront of stem cell and tumor biology. Cambridge, Mass.-agency initiative of the Broad Institute, where a full set of Fluidigm single-cell tools, processes and technology experiments, the most striking is C1 TM unicellular automatic preparation system and BioMark HD TM system.
technology
The single cell C1 TM FLUIDIGM automatic preparation system is based on the pioneering companies microfluidics, this technology allows researchers to quickly and reliably separated, and the processing described for the single cell genomic analysis. Scientists can use a technique to extract, reverse transcribe, amplify and ultimately detect and analyze cell activity, thereby reducing the differences between different platform technologies.
In this experiment a recently published article, used a total of five sets of single-cell C1 TM automatic preparation system, resulting in a biologically meaningful data from 1774 cells.
This article is published on the first single cell 10 C1 TM automatic document preparation system.
About Fuluda
Fuluda (NASDAQ: FLDM) develops, markets and markets life science analysis and preparation systems for high-growth markets such as single-cell biology and production genomics. We sell to the world's top research institutions, clinical laboratories, pharmaceutical companies, biotechnology companies and agricultural biotechnology companies. Our system is based on patented microfluidic and multi-parameter flow mass spectrometry technology. The product design greatly simplifies the experimental process, increases throughput and reduces cost, while providing excellent data quality. Fuluda products are for research use only. Not available for diagnostic steps.
FLUIDIGM®, C1TM and BioMarkTM are trademarks or registered trademarks of FLUIDIGM.
About the Broad Institute of MIT and Harvard University
The Eli and Edythe L. Broad Institute at MIT and Harvard University was established in 2004 to give creative scientists the opportunity to transform medicine. The Broad Institute aims to describe all the molecular components of life and their connections; to discover the molecular basis of major human diseases; to develop effective diagnostic and therapeutic methods; and to publish discoveries, tools, methods and data to the entire research community.
The Institute was established by MIT, Harvard University and their affiliated hospitals, as well as Los Angeles philanthropists Eli and Edythe L. Broad, and includes professors, professionals and students in the biomedical field at MIT and Harvard University. The Institute has partnerships with more than 100 private and public research institutions in more than 40 countries around the world. For more information on the Broad Institute, please visit http://
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