Pharmacological effects
1, the role of the cardiovascular system:
1 to increase myocardial nutrient blood flow: to psoralen, Cynomorium, Rhizoma Polygonati, dog's ridge, Dipsacus, red peony and other Chinese medicine composition, made of decoction, concentrated into 3g (raw medicine) / ml. Thirty-eight 18-22 g mice were used in the experiment, both male and female. Randomly divided into control group, administration group I, II group 10 each. The dosing group was intragastrically administered at 120 g/kg body weight. The control group was given the same volume of saline. 1.4 hours after administration, a 0.1 ml/kg body weight dose of RbCl solution (about 6.6-6.9×10 − cpm/0.1 ml) was injected from the tail vein of the mouse. The injection was completed within 2 minutes and the timing was immediately measured. Inject 0.2-0.3 ml of saturated KCl solution over 30 minutes. Animals were sacrificed, hearts were removed, and the myocardial 86Rb uptake rate was calculated using a LKBr counter to indicate myocardial nutrient flow, as shown in Table 6. The experimental results showed that the intragastric administration of compound Buguci powder for 1 hour could significantly increase the myocardial nutrient blood flow (P<0.01), but the administration time was extended to 4 hours and the increase of myocardial nutrient blood flow in mice had No statistical significance. The same method was used to determine the effect of compound Buguzhi granules on myocardial nutrient blood flow in aged mice. It was shown that compound Buguzhi granules can significantly increase the myocardial nutrient blood flow in aged mice (P<0.001), and the elderly group can The young group was close (P<0.05).
2 Protective effect of pituitrin-induced acute myocardial ischemia in mice: Thirty male mice weighing 18-22g were used both male and female, and were randomly divided into control group, pituitary Housu group and administration group each with 10 rats. In the control group and pituitary group, 1 g of physiological saline was administered for 2.5 hours, and the pituitary group and the administration group were injected intraperitoneally with 1 μg/kg body weight of pituitrin. After 30 minutes, the myocardial nutrient blood flow was measured in each group of mice. The results showed that compound Fuguzhi granules had a significant protective effect on acute myocardial ischemia induced by pituitrin in mice.
2, the role of the respiratory system:
Experiments showed that psoralen had a significant relaxation effect on histamine-induced tracheal contractions, with the strongest effect at 15 minutes after dosing, but slightly inferior to aminophylline. The differences between the two groups and the blank control group were compared. Very significant.
3, photosensitive effect:
1Inhibitory effect of psoralen plus black light therapy on common pathogenic fungi and bacteria caused by dermatosis: Use a 6 mm diameter filter paper tablet to add the solution (containing psoralen 1.0 mg/2.0 ml or isomeric psoralen) 2.0mg/2.0ml) 1.25, 2.5, 5.0, 10.0, 20.0?g. Leave 37°C in the drying oven for 1 hour. The fungus was inoculated with Sabber's medium flat dish, and bacteria were added to the nutrient agar flat dish. After the different concentrations of liquid filter paper paste on the flat disc of each strain. After the fungus was inoculated, it was preliminarily placed in the incubator at 26-28°C for 1 hour. The light was irradiated once every 10 minutes for 6-14 consecutive days. Bacteria were inoculated and placed in a 37°C incubator for 1 hour. The light was taken out for 10 minutes and incubated in a thermostat for 24 hours. Separately set up the drug-free solvent light group and the drug-free light group to observe for 1-7 days. The results showed that the psoralen agents plus black light irradiation had different degrees of bacteriostasis on the bacteria. There is a different zone of inhibition at the highest concentration of agent. In addition, Aspergillus is 8-15mm in the inhibition zone, 11-12 in the bacterium, 16-18 in the genus Mucor, and 10-16 in the genus Cephalosporium. In addition, it was proved that no antibacterial effect was observed in the non-irradiated and non-medicated groups.
2 isopsoralen plus black light irradiation skin graft rejection: 1 year and a half years old healthy rabbit weight 1.5-2.0kg, both male and female, each pair of donor and recipient gender. Divided into 5 groups: Group I (control group): after skin transplantation, no medication, lighting; Group II: medication and light on the following day after transplantation; Group III: medication and illumination on the fourth day after transplantation; Group IV: transplantation On the following day, he began to take medicine, not according to light; Group V: After the skin was transplanted, he began to shine light the next day and he did not take the medicine. Two 1.0 cm2 skin grafts were taken from the skin graft and sewn to the skin of the other rabbit. The distance between the two was 3.0 cm. Exchange skin grafts were exchanged between each group. The drug is an iso-pseudo-sulphone capsule, each containing 2.0 mg of isomeric psoralen. Each rabbit takes 1 capsule per night. The 20W domestic black light tube with 5 arrays was placed on the light, 5.0 cm from the transplant area, and each rabbit was irradiated with light for 30 minutes after taking the medicine for 2 minutes. The survival time of transplanted skin is shown in Table 11. The maximum observation time was 45 days. Observation of transplanted skin: Groups I, IV, and V showed skin paleness and softness 24 to 48 hours after transplantation; ruddy spots appeared on the third day; 4-5 days later, some skin patches appeared dark purple and hardened; margins had self-epidermal regeneration; 7 -3/4 skin patches darkened and necrosed on -9 days; self skins appeared below; skin slices were taken at 5-7 days and HE staining was performed. Microscopically, the dermis was clearly surrounded by lymphocytic infiltrate, small blood vessels. Congestion, endometrial shedding, thrombosis or necrosis. On the third day of group II and III, the skin was ruddy, soft, gradually thickened, and the normal skin condition was restored within 10 to 20 days. The edges of the skin grafts were observed for 25-30 days. HE staining was performed under a microscope. Peripheral lymphocytes, no other changes.
3 Activation of tyrosinase: 95% ethanol extract of psoralen significantly activates tyrosinase and increases the rate and amount of melanin production. Psoralen and isopsoralen can also promote the synthesis of melanin in the skin and deposit it under the skin.
4, the role of the immune system:
1 Fifteen female Wister purebred rats weighing 250-350 g were randomly divided into control groups (8
Psoralen medicinal effect
Psoralen medicinal effect
Only in the administration group (7), the administration group was fed with compound Buguzhi granules at a dose of 20 g/kg body weight (comprised of psoralen, Cynomorium osmophilus, Rhizoma Polygonati, schizophrenia, Dipsacus, Chishao, etc.). Made of decoction, concentrated to 3g (crude drug)/ml). The control group was given an equal volume of saline. Ten days later, 1 ml of blood was collected from the rat abdominal aorta and injected into a sterile heparin anticoagulant penicillin bottle. In a sterile room, the cellular immune function was determined in each group according to the improved 3H-TdR incorporation method. At the same time, the total number of white blood cells in rats was counted, and blood count was used to count the percentage of white blood cells. Experiments showed that compound Fukushima Granules can significantly increase the percentage of leucocytes in rats (P<0.02) and reduce the percentage of neutrophils, thus significantly enhancing rat cellular immunity. Function (P<0.01).
2 Kunming mice, weighing 18-22g, both male and female. A total of 4 groups were divided into 4 groups. The feeding conditions of the normal group were the same as others, but no drugs were used. The remaining 3 groups were intraperitoneally injected with 1 mg/g ethyl carbamate to induce lung adenocarcinoma. After the control was given carcinogen for 24 hours, the normal saline was injected intraperitoneally 0.2 ml. A total of 40 injections were performed 5 times a week and 16 weeks. In the psoralen extract group, 1 mg (0.2 ml)/20 g of psoralen was administered each time; in the cyclophosphamide group, 5 g (0.2 ml)/20 g of cyclophosphamide was administered each time. The number of administrations, time, and route of administration were the same as in the control group, and macrophage phagocytosis assays and E rosette test were performed after 16 weeks. Description psoralen has a significant role in enhancing the body's immune function.
5, anti-tumor effect: in vitro test, psoralen (250μg/ml) has a highly effective killing effect on mouse sarcoma cells, the mechanism is psoralen in the dark and sarcoma cells DNA complexes and molecular formation The chain inhibits DNA synthesis. After psoralen acts on human mucoepidermoid carcinoma MEC-1 cells, most of its cells have mitochondrial degeneration, vacuolization, free ribosomes, free ribosomes, and nuclear lysis.
6, anti-fertility and estrogen-like effects: female mice injected intrauterine 10mg isopsoralen or 0.00125-0.005ml bakuchiol, all have a significant anti-pregnancy effect, but the bakuchiol toxicity Stronger. Buguzhi powder has estrogen-like effects, can increase vaginal keratinization and increase uterine weight.
7, anti-aging effects: psoralen to varying degrees can significantly extend the silkworm larvae and silkworm life.
8, increase the role of white blood cells: animal experiments show that psoralen can promote the growth of granulocytes, and can protect animals after the injection of cyclophosphamide caused by leukopenia.
9. Other effects: Psoralea decoction has a killing effect on cysticerci, 40% concentration effect for 24 hours, can kill 88.O% of cysticerci, and can kill 47.5% when the concentration is 4%. 40% of psoralen decoction also has a strong killing effect against Trichomonas vaginalis. The effect of exogenous body can be eliminated within 30 minutes in vitro and the effect is stronger than that of rhubarb. In addition, psoralen has a hemostatic effect on a variety of hemorrhagic diseases (such as uterus, gums, and nose bleeding).
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