Answer: The radish split is the result of the expansion of the lateral root of the radish. The main reasons are several: First, the soil. Planting radishes must choose deep and well-drained plots. If there are hard objects such as stones in the soil layer, it will hinder the growth of radish roots, which will cause the lateral roots of the radish to expand and bend, causing the radish to fork. The second is the cause of moisture. Radish needs sufficient water during the growth process, but it should not be too high. If the water is high for two days, it will cause poor soil aeration, inhibit the main root of the radish and form a lot of lateral roots, so the drainage work must be done well. The third is the reason for fertilization. Radish fertilization, especially farmyard manure, must be thoroughly decomposed, and unfertilized farmyard manure can cause radish root malformation. The fourth is the reason for the temperature. Radish must pay attention to the shed film before sowing, keep the temperature suitable, otherwise it will easily cause the radish fork. The fifth is the cause of pests. Underground pests will eat radish, inhibit the growth of the main roots, causing lateral root enlargement, bifurcation or deformity.
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The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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