Biologists use the FFU laminar flow hood to prepare agar plates
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Molecular Biology and Bacteriology Agar plates are used to culture microorganisms, such as bacterial experiments. Agar solidification agent, or gel,
Nutrient medium required for the survival and growth of liquid microorganisms. Once transferred to an agar plate, the bacteria grow into different colonies
The population (if grown at low concentrations) or smear (if at high concentrations) can be harvested for analysis. Biologist prepares agar
For plates, this process requires access to laboratory-level reagents and a good understanding of the machine and molecular biology and aseptic techniques.
1. Put on appropriate protective equipment, including gloves, lab coats, masks, and purify all surfaces. Working in the Bunsen burner, Zui is good
Can flow hood in a sterile FFU layer. This prevents air pollutants from falling into the agar.
2. Remove the stacked petri dish packaging except for the reloaded agar plate. Label the outer surface of the dish (cover) and agar
Type and date preparation. The plate comes out as a separate layer with a lid to remove the simplified pouring agar.
3. Mix the trypsin, yeast extract and agarose together, then add the sodium hydroxide (sodium hydroxide) solution.
Add 1 x nutrient medium by adding sterile distilled water. This solution was transferred to a glass bottle with a volume of at least 1.5 liters.
Loose screw caps for bottles and labels for date preparation and media names. Autoclave tape in bottles and autoclave for 25 minutes
. Allow the bottle to cool to no less than 45 degrees Celsius (or harden too early) but no more than 50 degrees Celsius. Can store bottle cooling and
Solidify the agar at 4 degrees Celsius and then warm until liquefied. If you need a specific experiment or strain of bacteria, add the appropriate class
Type and quantity of supplements such as antibiotics. If the media is too hot, the supplement will be destroyed, so double check the temperature before the media
degree.
4. Pour a sufficient amount of agar into the plate and the agar is still warm and liquefied. Make sure the surface is completely covered. Don't fall over
Or let the agar of this dish reach its peak. Pass the plate in a sterile atmosphere by allowing the Bunsen burner or laminar flow cover to continue to run
. Allow the panel to be set and then replace the cover.
5. Dry the agar plate for 30 minutes to an hour in the oven no higher than 37 degrees Celsius. Or you can have them flow in the FFU layer
Cover for three minutes or in a low flow area that is not air permeable in the purification laboratory, one or two days. Dry tray stops any bacterial solution placement
On agar surface from simple dilution and sliding instead of forming colonies, stick to agar.
6. Carefully store the disk in the dark at 4 degrees Celsius. Stack and dry cup of agar plate let you clearly see the label and the defense
Stop microbes and debris falling on agar surface contamination. Put the plates back to their packaging, cover and foil tape to close the package.
Prepare the packaging board with their content and date labels. You can store the disk for up to two months and you should abandon them.
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