Dimethyl sodium bicarbonate (DMDC) treatment kit product manual
The main purpose
Dimethyl sodium bicarbonate (DMDC) treatment reagent is a novel technical method designed to inhibit or eliminate RNase contamination and replace diethylpyrocarbonate (DEPC). The technology has been carefully developed and successfully tested. It is suitable for experimental work on all RNA. The product is ready to use, stable performance, convenient operation and satisfactory results.
technical background
Dimethyl dicarbonate (DMDC) is a chemical inhibitor of RNase. As a food preservative and additive, it is non-toxic and has no residue. It is an ideal substitute for the treatment of RNA interference factors by diethylpyrocarbonate (DEPC). In addition, the operation is more simple than the DEPC.
product content
Treatment solution (Reagent A) 10 ml
Product manual 1 copy
storage method
Stored in a -20 ° C refrigerator, effective for June
User-supplied
1 liter or 2 liter beaker: container for operation
Heating mixer: for mixing solution or removing DMDC
High pressure steam sterilizer: used to remove DMDC
Experimental procedure
- Prepare a 1 liter or 2 liter beaker
- Add the solution required by the user
- Add 1 part of the treatment solution (Reagent A) to the beaker of 100 parts of solution (1:100 ratio)
- Mix thoroughly
- Incubate for 30 minutes at room temperature
- Subsequent high temperature processing:
- High-pressure disinfection solution or vessel: put into high-pressure steam sterilizer for 15 minutes at high temperature
- Solution or vessel that cannot be autoclaved: heated to 95 ° C and stirred for 15 minutes.
- Or a solution or vessel that cannot be autoclaved: heated to 70 ° C and heated for 1 hour.
7. Can be used after cooling
Precautions
- This product is 10 operations (100 mA / time)
- Wear gloves when handling
- The ratio of the capacity of the solution to be treated and the treatment solution (Reagent A) is 100:1.
- Steam heat treatment for 15 minutes to ensure no DMDC residue in the solution.
- The buffer contains amines, such as Tris, sulfhydryls, and dithiothreitol (DTT), which will react with sodium dimethylcarbonate to rapidly degrade DMDC. Therefore, it is necessary to add amines, including TE buffer, etc., after DMDC treatment.
- The company provides a series of RNA processing reagent products
Quality Standard
- This product has been certified to be stable.
- This product has been proven to be effective
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