Release date: 2017-07-18
The School of Optoelectronics of Shenzhen University recently announced that the team of academicians of the school, together with Tsinghua University, Zheyuan Technology, the University of Hong Kong, Lemon Data, and the University of Texas at Dallas, have independently built 3D random optical reconstruction microscopes (3D). On the -STORM) platform, a diagnostic optical imaging-based diagnostic tool was developed to obtain 3D super-resolution images of DNA sequences of only 2,500 bases in the context of a complex nuclear environment. This is an image of the shortest specific DNA sequence observed so far in the nucleus, and the related work was recently published in the international high-level open journal eLife.
Professor Zhang Qiwei, a co-author of Tsinghua University and the University of Texas at Dallas, said that the traditional FISH method is limited by various factors (including labeling ability and optical resolution) and it is difficult to obtain clear microscopic images of specific short fragments in the genome.
“Although this technique is promising in the study of the fine organization of chromatin domains, we hope to develop a new method: not only to further improve the resolution of the genome sequence, but also to avoid the cumbersome probe preparation process to the actual The limitations of the application, Professor Zhang Qiwei said, this new method can accurately target gene enhancers and promoters, interact, visualize in situ, and can be used to detect specific DNA in single cells in cancer and other diseases. Small fragments and changes in their interactions.
One of the authors, Dr. Cao Bo from Shenzhen University, introduced the new method of molecular beacon (MB) probes (MB-FISH) on the self-built 3D-STORM platform. The ability to display the distribution of small fragments of target DNA in the three-dimensional space within the nucleus under nano-resolution conditions is similar to the precise identification of the person in the hall of a crowd of more than 1 million people.
According to reports, the team is currently building and improving multi-color 3D-STORM, which will release greater potential in studying the 3D genomic structure of human normal and disease (eg cancer) cells.
The young scholars of the School of Optoelectronics of Shenzhen University who participated in the relevant work are all members of the team of the academician of the school. Niu Yuqian, who was elected as an academician of the Chinese Academy of Engineering in 1997, is an outstanding expert in optoelectronics and ultra-fast diagnostic technology in China. In 1999, he led his research team to Shenzhen University and became the first academician to be introduced by Shenzhen University.
In July 2016, the academician Niu Wei stupid died of illness. Qu Lejun, dean of the School of Optoelectronics of Shenzhen University, said that Niu Wei’s academician has been committed to the innovation of advanced optical methods and actively promoted the application of optical methods in life sciences. The release of this achievement is the best commemoration of the academician of the cow.
Source: China Youth Daily
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