Have you encountered these problems when grinding animal tissue? 301 hospital to demonstrate for you
Experimental site: General Hospital of the People's Liberation Army (301 Hospital)
Test sample: tissue of six parts of heart, liver, spleen, lung, kidney and brain of young pigs
Experimental purpose: analysis of drug metabolism
Grinding step:
1. Take out the heart, liver, spleen, lung, kidney and brain tissue samples of the young pigs placed in the refrigerator at -20 °C for a little thawing. Take 2 samples of each part as a set of controls, cut into small pieces and set them separately. In a 2 ml centrifuge tube, number and place in an ice box.
2. Each grinding will be carried out in units of the control group. Taking spleen grinding as an example: Transfer 2 spleen tissue samples to 5 ml hard plastic grinding tubes matched with 2 Dingyuan TL1000 small tissue grinders. Add 8 mm stainless steel beads to each tube, and add 1 ml to one tube. Phosphate tissue fluid, add 1ml ammonium acetate tissue solution to another tube, cover the tube cover, and install the machine;
3, using automatic intermittent grinding method, 2000 rpm, set 2 cycles, each cycle 60s, cycle interval 10s, after the completion of grinding, use a pipette to transfer back to the corresponding number of 2ml centrifuge tube;
4, due to the different toughness of the visceral tissue samples, the grinding of the liver and brain tissue, using only one method of grinding for 90s, the grinding time is shortened, but the grinding effect is still very good; for the grinding of the heart tissue, the use of Ding Wei The stainless steel grinding tank of the source TL1000 small tissue grinder makes the grinding more uniform.
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