C13C4.5/Spinster, an evolutionarily conserved protein that acts on fat metabolism and regulates nematode fertility

Union BioMetrica COPAS Large Particle Flow System Application

C13C4.5/Spinster, an evolutionarily conserved protein that acts on fat metabolism and regulates nematode fertility

C13C4.5/Spinster, an evolutionarily conserved protein that regulates fertility inC. elegans through a lysosome-mediated lipid metabolism process

Mei Han, Hao Chang, Peng Zhang, Tao Chen, Yan Hua Zhao, Yongdeng Zhang, Pingsheng Liu, Tao Xu, Pingyong Xu

Protein & Cell May 2013, Volume 4, Issue 5, pp 364-372

Professor Xu Tao from the Institute of Biophysics of the Chinese Academy of Sciences used the Union BioMetrica COPAS large particle flow system to publish in the internationally renowned journal " Protein and Cell " this year - C13C4.5/Spinster, an evolutionarily conserved protein that acts on fat metabolism. Regulate the fertility of nematodes. (Protein & Cell, May 2013, Volume 4, Issue 5, pp 364-372).

Lipid droplets exist in almost all species of organelles, whose main function is to store neutral fat. Understanding the mode of regulation of lipid droplets will help to address obesity and other fat-related diseases. In this study, the researchers selected 11 candidate RNAi control sequences that may be associated with lipid metabolism in Caenorhabditis elegans. Using the Union BioMetrica COPAS large particle flow cytometer with the BODIPY 493/503 laser for analysis, the researchers found that six of the candidate sequences were negatively controlled for fat storage, and three candidates were positively controlled for fat storage. The investigator selected one of the negative regulatory RNAi C13C4.5 as a follow-up study.

original

C13C4.5 is mainly expressed in the intestinal tract of nematodes. The researchers found that C13C4.5 is an important gene for maintaining lipid droplet metabolism. Biochemical analysis revealed that 50% of the triacylglycerol (TAG) was lost in the C. cerevisiae knockout C13C4.5 gene. The stimulated Raman scattering (SRS) signal showed that the C13C4.5 mutant had a 49.6% fat content in the proximal region of the intestine compared to the wild type, while the wild type 86.3% had a fat content in the anterior region of the intestine. Compared with the wild type, the C13C4.5 knockout nematode showed a significant decrease in the size of the lipid droplets and the signal intensity. The membrane structure of the LMP-1 marker also increased in the intestinal tract of the C13C4.5 mutant nematode. Fertility defects were also found in the final C13C4.5 (ok2087) mutant. Taken together, these results suggest that C13C4.5 may regulate the fat content and size of nematode lipid droplets by interfering with the morphology and function of lysosomes, and indirectly regulate fertility.

Model animals such as nematodes, zebrafish, model plant seeds, large-volume cells (stem cell clusters, 3D cell teams) and microsphere sorting have a very wide range of applications in life science research, but because these objects are too large, Ordinary flow cytometry is difficult to sort, and manual mirroring is time-consuming, inefficient, and difficult to guarantee. Union Biometrica's COPAS worm/embryo flow sorting system/multi-parameter biological particles The analysis and sorting system is the only flow cytometer on the market that can analyze and sort large particles.