The correct diagnosis of fish diseases is the basis of effective prevention and treatment of diseases, especially in the early diagnosis of fish diseases. Whether or not the medicine can be prescribed to the disease, whether it can make the medicine effective, and whether it can effectively control the spread of the disease depends on the diagnosis of fish diseases. .
First, collect samples
The diseased fish were selected as the object of diagnostic tests if the diseased fish were found to have died of sudden illness or shortly after death. For diseases that cannot be diagnosed immediately, the samples should be transported to a professional laboratory for inspection by fresh transport (4°C). Samples are very important for the rapid diagnosis of fish diseases. If further diagnostic analysis is required, fixatives can be used to fix and preserve diseased fish or internal organs.
Sampling pond water samples taken at multiple sampling points to take the fish pond water samples (50 to 80 cm below the water surface), immediately sent to the professional laboratory analysis.
Second, on-site investigation
1. Species structure, seed source, size, health status, and stocking density of cultured species.
2. Disinfection of pond ponds and daily preventive measures, drugs used, and methods of use.
3. Feed the type, source, feeding method, feeding amount, etc. of the feed.
4. Health and health management measures in the breeding process. Water source, water quality, water temperature, sediment quality, etc.
5. Onset and non-disease activity of fish in aquaculture ponds, such as swimming and feeding.
6. Incidence and the measures taken, including the time of onset, type of illness, illness and condition, deaths, measures taken and results, etc.
7. The pond area, sediment quality, water depth, water color, transparency and so on.
Third, pathogen identification
1. Visual inspection Visually observe whether the color of affected fish and organs (including ticks) changes with inflammation, congestion, hemorrhage, anemia, swelling, ulcer, mucus, ascites, etc., and whether or not there is any foreign matter. Visually observe the presence of fungal or parasitic parasites in the affected fish.
(2) The microscopic inspection uses tweezers to scrape the mucous of external organs such as skin, fins, and gills, or to cut a part of diseased tissues such as filaments and fins with ophthalmology, and prepare water-impregnated tablets on glass slides. Under a microscope, check for fungal or parasitic parasites and take pictures to observe the results if necessary.
3. Anatomic examination of newly diagnosed diseased fish, visual observation of changes in the color and shape of internal organs, such as inflammation, congestion, bleeding, swelling, ulcers, atrophy, hypertrophy, body cavity water and other pathological changes, with or without Parasites and their cysts.
4. Pathological biopsy Take a small piece of diseased tissue or organ. After the procedure of fixation, dehydration, embedding, etc., the sample is sectioned and stained with the corresponding staining method to show the changes of different cells and cells. Optical or electron microscopy (this method needs to be performed in a professional laboratory).
5. PCR instrument detection PCR instrument detection technology has been recommended by the World Animal Health Organization as one of the diagnostic methods for some fish diseases. Its main principle is to identify pathogenic organisms and diagnose diseases by designing specific primers to amplify specific gene fragments of pathogenic organisms (this method needs to be performed in specialized laboratories).
Four, symptomatic treatment
In the diagnosis of fish diseases, the cause of the disease should be analyzed and the possibility of compound or secondary infection should be considered. After determining the main causes and secondary causes, symptomatic treatment should be performed.
Nuts Seed Kernel include walnut kernel, sunflower seeds kernel, sunflower seed kernel etc., which are excellent food with rich nutrition.
Seed Kernel,Kernel Fruit,Walnut Kernel,Pumpkin seed kernel, sunflower seed kernel
Xi'an Gawen Biotechnology Co., Ltd , https://www.agolyn-bio.com