Ultra-clean bench principle, use and maintenance The ultra-clean workbench is a facility that provides a sterile operating environment for laboratory work to protect the experiment from the external environment while providing some degree of protection to the external environment from contamination. Protect the operator. Compared to a simple sterile cover, the clean bench has the advantage of allowing the operator to move freely, easily reach the operating area and with higher safety. At present, the ultra-clean workbench used in China has imported products, such as BIOGARD B40-112, which is a super-clean workbench that meets the US Class A safety standard. It is a kind of organism that tends to have low and medium risk. Learn the products recommended for testing.
(a) Principle
The clean environment of the clean bench is formed by flowing clean air (inlet air) in a specified direction in a specific space. Divided by the direction of the airflow, the existing ultra-clean workbench can be divided into vertical, from the inside to the outside and from the side. Considering the quality of operation and the impact on the environment, it is superior in vertical mode. The clean air supplied by the air supply filter plate descends through the operation area at a specific speed, is separated in the middle of the operation area, is sucked away by the front air suction hole and the rear suction window, and the air sucked in the front and rear portions at the lower portion of the operation area is mixed. Together, and pumped into the rear positive pressure zone by a blower, in the upper part of the machine, 30% of the gas is discharged from the top through the exhaust filter plate, and approximately 70% of the gas is re-entered into the operating zone through the oxygen supply filter plate. To supplement the air discharged from the exhaust port, the same volume of air is replenished from the room air through the operating port. This air will never enter the operating area, just forming an air barrier.
Many domestic ultra-clean workbench only have air supply filter plates, and the filtered air enters the operation area to form a certain positive pressure. The air is discharged into the ambient air from the set vent hole and the operation port. This air flow mode is applied to the surrounding environment and operation. None of them have a protective effect.
(2) Commissioning
The ultra-clean workbench is rigorously tested before leaving the factory to ensure normal use. But this is not to say that the manufacturer's test can replace the operator to perform the necessary inspection and debugging before use. Because the environment in which the ultra-clean workbench is located is different, only the proper inspection and debugging can maximize the role of the equipment.
A better environment should be selected for the machine before commissioning. Place it in a sterile room with an air disinfection facility. If conditions are not available, place the machine in a clean, clean room. Adjust the height of each foot to ensure a safe and level of operation. The power supply of the ultra-clean workbench should use a special circuit to avoid the change of air flow rate caused by the overload of the circuit.
Ultraviolet germicidal lamps and fluorescent lamps are standard on ultra-clean benches . The blowers provide power for air flow. It is clear at a glance whether these components are working properly. The most difficult and important thing is to inspect the air filter plate and its tightness, which is directly related to the normal use of the machine. The simplest method of examination is the nutrient agar plate method.
The newly purchased and unused ultra-clean workbench can be fumigated in addition to ultraviolet light, etc., and then an open nutrient agar plate is placed in the four corners and the center of the operation area when the machine is in operation. After the hour, the lid was placed and placed in a 37 ° C incubator for 24 hours to calculate the number of colonies. The average number of colonies per plate must be less than 0.5.
(3) Use
The ultra-clean workbench is irradiated with UV light for 30 to 40 minutes before use, and the various open doors and windows around the operating area are inspected for working position. The operation takes place at the center of the operating area, which is a safer area in design.
You should have a preliminary understanding of the experimental materials before proceeding, and understand the performance and safety level of the equipment you are using. Strictly enforce laboratory safety procedures. The use of specific pathogens in any ultra-clean workbench must be evaluated for safety. If the test material causes environmental pollution to the surrounding environment, it should be avoided in the model without the exhaust filter, because operating in flowing air is no different from dispersing.
Any advanced equipment does not guarantee the success of the experiment. The use of the ultra-clean bench of the animal quarantine laboratory is aimed at sterility and avoiding cross-contamination, so skilled operation and clear sterility essentials are essential.
(four) maintenance
The ultra-clean workbench is a relatively sophisticated electrical device that is very important for regular maintenance and maintenance.
First of all, we must keep the indoors dry and clean. The humid air will not only rust the manufacturing materials, but also affect the normal operation of the electrical circuit. The humid air is also conducive to the growth of bacteria and mold. A clean environment also extends the life of the filter plate.
In addition, regular cleaning of equipment is an important part of normal use. Cleaning should include routine cleaning before and after use and regular fumigation. When fumigation, all gaps should be completely sealed. Cleaning should include routine cleaning before and after use and regular fumigation. When fumigation, all gaps should be completely sealed. For example, the type of ultra-clean workbench with movable baffle cover at the operation port can be sealed with plastic film.
The filter plates and UV germicidal lamps of the ultra-clean workbench have a calibrated service life and should be replaced on schedule.
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