In the above case, the enzyme sample is acidified, resulting in protein denaturation... White ignores the effect of dry ice sublimation on CO 2 on the sample solution of the enzyme stored in the common sample tube... Usually, even for short-term storage, the sample may Acidified by the influence of CO 2 . This acidification affects the integrity and renaturation of the sample and may result in loss of activity. In this case, a special anti-CO 2 tube is used to store the sample.
The VWR anti-CO 2 tube is effective in protecting the sample from acidification, and other containers cannot protect the sample during the sublimation of dry ice to CO 2 . The formation of carbonic acid will result in a pH reduction of 2.5 pH units. In this experiment, the acidification performance is the color of the reagent from blue to yellow.
Let's take a brief look at the following concepts:
Protein denaturation
It refers to the specific spatial conformation of a protein under certain physical and chemical factors, resulting in changes in its physical and chemical properties and loss of biological activity. This phenomenon is called protein denaturation.
How to prevent protein denaturation?
Low temperature
2. Suitable pH value
3. Suitable salt concentration
4. Avoid vigorous stirring or shaking
The VWR anti-CO 2 tube protects expensive proteins, and cell samples are not destroyed by CO 2 acidification of dry ice gasification. The tube is made of durable, high-quality material with a sealing cap, a protective barrier against CO 2 tubes, impervious to CO 2 , keeps the sample pH stable, suitable for dry ice transport and storage
Let us briefly understand the anti-CO 2 ability of VWR anti-CO 2 tube through CO 2 resistance test.
experiment
The solution with a pH of 8.0 was pre-frozen and placed on dry ice for 48 hours to measure the pH.
[Other tubes] Carbon dioxide enters the top of the competitor tube, reducing the pH of the protein solution
[VWR anti- CO 2 tube] Only anti-CO 2 tube can protect the solution from acidification
At present, VWR provides you with two sizes of anti-CO 2 tubes, the capacity is 15ml and 50ml respectively, let us take a quick look
The sample in the anti- CO 2 tube should be frozen before contacting dry ice.
About VWR China
As a long-standing commitment to pharmaceutical, biotechnology and healthcare, VWR is a quality supplier of cleanroom products, services and solutions for laboratories and manufacturing companies.
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