Copper protein electrophoresis staining kit product manual (Chinese version)
The main purpose
YIJI Copper Electrophoresis Staining Reagent is an authoritative method for dyeing directly on polyacrylamide gels using sodium diethyldithiocarbamate staining agent to produce a protein brown band with a sensitivity of 50 ng or more on a clear background. Classic technical method. The technology has been carefully developed and successfully tested. It is suitable for the specific detection of copper protein electrophoresis. The product is ready to use, stable performance, convenient operation, high sensitivity, clear color, good repeatability, and establish a standardized system.
technical background
A copper protein is a group of proteins containing one or more copper ions as a prosthetic group. It is divided into type I, type II, type III, and the like. Type I is a common copper binding protein, such as plastocyanin, azurin, and the like. A sodium diethyldithiocarbamate (SDTC) stain was used to selectively bind copper ions and display brown. It is commonly used for the detection of copper protein gel electrophoresis.
product content
YIJI staining solution (Reagent A) ml
1 product manual
storage method
Store at room temperature; YIJI staining solution (Reagent A), avoiding light, effectively guaranteeing June
User-supplied
Plastic tray: container for dyeing
Ion-free water: a solution used to dilute reagents
Flat shaker: for incubation during dyeing operations
Experimental procedure
Before the start of the experiment, the SDS-PAGE protein electrophoresis was completed, and the colloid of 6 cm X 8 cm was taken out, and the orientation mark was made, and then the following operations were performed:
- Prepare a stained plastic tray of 8cm X 10cm size
- Put in polyacrylamide gel
- Add xx ml of YIJI staining solution (Reagent A) on the gel to cover the entire colloid
- Incubate for 16 hours at room temperature on a flat shaker at 50 rpm to avoid light
- Carefully pour off the staining solution in the staining tray
- (Selection step) Add 50 ml of user-supplied ion-free water and shake it by hand for 10 seconds.
- (Selection step) Carefully pour off the ion-free water
- (Selection step) Immediately under the imager for observation and photography: the strip shows brown
Precautions
- This product is 10 operations
- Each 25 ml kit solution is used to treat 6cm X 8cm size colloid; every 50ml kit solution is used to treat 12cm X 12cm size colloid; the larger the colloid, the increased the amount
- Wear gloves when handling
- Incubate at room temperature to avoid drying out
- Immediately after incubation, observation; placement time is too long, affecting the effect
- The company provides a series of protein dyeing reagent products
Quality Standard
- This product has been certified to be stable.
- This product has been identified and clearly colored
The sampling spoon and the lid are injection-molded together, making sampling more reliable. Larger matte surface for easy writing and marking by inspectors.
Good sealing can effectively prevent leakage and contamination of specimens, facilitate storage and transportation, avoid contact between patients, medical staff, and specimens, and is safe and reliable.
Labels can be customized according to customer needs.
Different sterilization methods available: EO, irradiated, or non-sterile
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